detection antibodies rat anti mouse ige biot (SouthernBiotech)
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detection antibodies rat anti mouse ige biot
Detection Antibodies Rat Anti Mouse Ige Biot, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rat+ig+antibody/Rat+Anti-Mouse+IgE-BIOT/bio_rxiv__64898__2026__02__23__707402-227-13-18
Average 93 stars, based on 21 article reviews
Detection Antibodies Rat Anti Mouse Ige Biot, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+rat+ig+antibody/Rat+Anti-Mouse+IgE-BIOT/bio_rxiv__64898__2026__02__23__707402-227-13-18
Average 93 stars, based on 21 article reviews
detection antibodies rat anti mouse ige biot - by Bioz Stars,
2026-09
93/100 stars
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Labeling:Article Title: PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade Article Snippet: Cells were washed and stained with anti-PD-L1 mAbs (5A2-A1, rat IgG 1 ; 6C11-3A11; rat IgG 2a ) [ , ] at room temperature for 30 min. Rat IgG 1 (R3-34, BD Biosciences) and rat IgG 2a isotype controls (R35-95, BD Biosciences) were used for negative control staining. .. Cells were washed with PBS containing 1% BSA (Sigma–Aldrich) and labeled with APC-conjugated Article Title: Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle. Article Snippet: .. Target isotype clone source Fluorochrome conjugation or labeling Binding assay of anti-programmed death-1 (PD-1) antibodies PD-1 Bovine IgG1 Boch5D2 This study Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) Bovine IgG1 isotype control Bovine IgG1 Poly Bethyl Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) PD-1 Rat IgG2a 5D2 In house (25) APC APC-conjugated Article Title: Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle Article Snippet: .. 688 on S eptem ber 3, 2016 by C O R N E LL U N IV E R S IT Y http://iai.asm .org/ D ow nloaded from TABLE 1 Primary MAb used in flow cytometric analyses for this study _______________________________________________________________________________________________________ Target Isotype Clone Source (reference) Fluorochromea Conjugation or labeling _______________________________________________________________________________________________________ PD-1 and LAG-3 CD4 Mouse IgG2a CC8 AbD Serotec FITC Conjugated primary antibody CD8 Mouse IgG2a CC63 AbD Serotec FITC Conjugated primary antibody TCR1-N24 Mouse IgG2b GB21A WSU MAb Center FITC FITC-anti-mouse IgG + IgM (δ chain) (H + L) antibody (Caltag Laboratories) CD3 Mouse IgG1 MM1A WSU MAb Center PerCp-Cy5.5 Lightening-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences) PD-1 Rat IgG2a 5D2 In house (11) APC APC-conjugated anti-rat IgG antibody (Southern Biotech) Rat IgG2a Rat IgG2a R35-95 BD Biosciences APC APC-conjugated anti-rat IgG antibody isotype control (Southern Biotech) LAG-3 Rat IgG1 71-2D8 BD Biosciences PE PE-conjugated anti-rat IgG1 antibody (Southern Biotech) Rat IgG1 Rat IgG1 R3-34 BD Biosciences PE PE-conjugated anti-rat IgG1 antibody isotype control (Southern Biotech) PD-L1 CD-14 Mouse IgG1 CAM36A WSU MAb Center PerCp-Cy5.5 Lightening-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences) PD-L1 Rat IgG2a 4G12 In house (16) APC APC-conjugated Article Title: Cooperation of PD-1 and LAG-3 in the exhaustion of CD4 + and CD8 + T cells during bovine leukemia virus infection Article Snippet: PBMCs were incubated in PBS containing 10% goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 15 min to prevent nonspecific reactions. .. Cells were then washed and stained with anti-PD-1 monoclonal antibody (mAb) (5D2, rat IgG 2a ) [ ] or rat IgG 2a isotype control (R35-95, BD Biosciences, San Jose, CA, USA) for 20 min at room temperature, washed with PBS containing 1% bovine serum albumin (BSA; Sigma–Aldrich), and labeled with APC-conjugated Article Title: PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade Article Snippet: Cells were washed and stained with anti-PD-L1 mAbs (5A2-A1, rat IgG 1 ; 6C11-3A11; rat IgG 2a ) [ , ] at room temperature for 30 min. Rat IgG 1 (R3-34, BD Biosciences) and rat IgG 2a isotype controls (R35-95, BD Biosciences) were used for negative control staining. .. Cells were washed with PBS containing 1% BSA (Sigma–Aldrich) and labeled with APC-conjugated FACS:Article Title: PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade Article Snippet: Cells were washed and stained with anti-PD-L1 mAbs (5A2-A1, rat IgG 1 ; 6C11-3A11; rat IgG 2a ) [ , ] at room temperature for 30 min. Rat IgG 1 (R3-34, BD Biosciences) and rat IgG 2a isotype controls (R35-95, BD Biosciences) were used for negative control staining. .. Cells were washed with PBS containing 1% BSA (Sigma–Aldrich) and labeled with APC-conjugated Article Title: PD-L1 expression in equine malignant melanoma and functional effects of PD-L1 blockade Article Snippet: Cells were washed and stained with anti-PD-L1 mAbs (5A2-A1, rat IgG 1 ; 6C11-3A11; rat IgG 2a ) [ , ] at room temperature for 30 min. Rat IgG 1 (R3-34, BD Biosciences) and rat IgG 2a isotype controls (R35-95, BD Biosciences) were used for negative control staining. .. Cells were washed with PBS containing 1% BSA (Sigma–Aldrich) and labeled with APC-conjugated Conjugation Assay:Article Title: Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle. Article Snippet: .. Target isotype clone source Fluorochrome conjugation or labeling Binding assay of anti-programmed death-1 (PD-1) antibodies PD-1 Bovine IgG1 Boch5D2 This study Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) Bovine IgG1 isotype control Bovine IgG1 Poly Bethyl Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) PD-1 Rat IgG2a 5D2 In house (25) APC APC-conjugated Article Title: Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle Article Snippet: .. 688 on S eptem ber 3, 2016 by C O R N E LL U N IV E R S IT Y http://iai.asm .org/ D ow nloaded from TABLE 1 Primary MAb used in flow cytometric analyses for this study _______________________________________________________________________________________________________ Target Isotype Clone Source (reference) Fluorochromea Conjugation or labeling _______________________________________________________________________________________________________ PD-1 and LAG-3 CD4 Mouse IgG2a CC8 AbD Serotec FITC Conjugated primary antibody CD8 Mouse IgG2a CC63 AbD Serotec FITC Conjugated primary antibody TCR1-N24 Mouse IgG2b GB21A WSU MAb Center FITC FITC-anti-mouse IgG + IgM (δ chain) (H + L) antibody (Caltag Laboratories) CD3 Mouse IgG1 MM1A WSU MAb Center PerCp-Cy5.5 Lightening-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences) PD-1 Rat IgG2a 5D2 In house (11) APC APC-conjugated anti-rat IgG antibody (Southern Biotech) Rat IgG2a Rat IgG2a R35-95 BD Biosciences APC APC-conjugated anti-rat IgG antibody isotype control (Southern Biotech) LAG-3 Rat IgG1 71-2D8 BD Biosciences PE PE-conjugated anti-rat IgG1 antibody (Southern Biotech) Rat IgG1 Rat IgG1 R3-34 BD Biosciences PE PE-conjugated anti-rat IgG1 antibody isotype control (Southern Biotech) PD-L1 CD-14 Mouse IgG1 CAM36A WSU MAb Center PerCp-Cy5.5 Lightening-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences) PD-L1 Rat IgG2a 4G12 In house (16) APC APC-conjugated Binding Assay:Article Title: Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle. Article Snippet: .. Target isotype clone source Fluorochrome conjugation or labeling Binding assay of anti-programmed death-1 (PD-1) antibodies PD-1 Bovine IgG1 Boch5D2 This study Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) Bovine IgG1 isotype control Bovine IgG1 Poly Bethyl Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) PD-1 Rat IgG2a 5D2 In house (25) APC APC-conjugated Control:Article Title: Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle. Article Snippet: .. Target isotype clone source Fluorochrome conjugation or labeling Binding assay of anti-programmed death-1 (PD-1) antibodies PD-1 Bovine IgG1 Boch5D2 This study Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) Bovine IgG1 isotype control Bovine IgG1 Poly Bethyl Alexa Fluor 647 Alexa Fluor 647-conjugated anti-bovine IgG Fc antibody (Jackson ImmunoResearch) PD-1 Rat IgG2a 5D2 In house (25) APC APC-conjugated Article Title: Cooperation of PD-1 and LAG-3 Contributes to T-Cell Exhaustion in Anaplasma marginale-Infected Cattle Article Snippet: .. 688 on S eptem ber 3, 2016 by C O R N E LL U N IV E R S IT Y http://iai.asm .org/ D ow nloaded from TABLE 1 Primary MAb used in flow cytometric analyses for this study _______________________________________________________________________________________________________ Target Isotype Clone Source (reference) Fluorochromea Conjugation or labeling _______________________________________________________________________________________________________ PD-1 and LAG-3 CD4 Mouse IgG2a CC8 AbD Serotec FITC Conjugated primary antibody CD8 Mouse IgG2a CC63 AbD Serotec FITC Conjugated primary antibody TCR1-N24 Mouse IgG2b GB21A WSU MAb Center FITC FITC-anti-mouse IgG + IgM (δ chain) (H + L) antibody (Caltag Laboratories) CD3 Mouse IgG1 MM1A WSU MAb Center PerCp-Cy5.5 Lightening-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences) PD-1 Rat IgG2a 5D2 In house (11) APC APC-conjugated anti-rat IgG antibody (Southern Biotech) Rat IgG2a Rat IgG2a R35-95 BD Biosciences APC APC-conjugated anti-rat IgG antibody isotype control (Southern Biotech) LAG-3 Rat IgG1 71-2D8 BD Biosciences PE PE-conjugated anti-rat IgG1 antibody (Southern Biotech) Rat IgG1 Rat IgG1 R3-34 BD Biosciences PE PE-conjugated anti-rat IgG1 antibody isotype control (Southern Biotech) PD-L1 CD-14 Mouse IgG1 CAM36A WSU MAb Center PerCp-Cy5.5 Lightening-Link PerCp/Cy5.5 conjugation kit (Innova Biosciences) PD-L1 Rat IgG2a 4G12 In house (16) APC APC-conjugated Article Title: Anti-Bovine Programmed Death-1 Rat–Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle Article Snippet: PD-1 , Rat IgG 2a , 5D2 , In house ( ) , APC , APC-conjugated anti-rat Ig antibody (Southern Biotech). .. Rat IgG 2a isotype control , Rat IgG 2a , R35-95 , BD Biosciences , APC , APC-conjugated Article Title: Cooperation of PD-1 and LAG-3 in the exhaustion of CD4 + and CD8 + T cells during bovine leukemia virus infection Article Snippet: PBMCs were incubated in PBS containing 10% goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 15 min to prevent nonspecific reactions. .. Cells were then washed and stained with anti-PD-1 monoclonal antibody (mAb) (5D2, rat IgG 2a ) [ ] or rat IgG 2a isotype control (R35-95, BD Biosciences, San Jose, CA, USA) for 20 min at room temperature, washed with PBS containing 1% bovine serum albumin (BSA; Sigma–Aldrich), and labeled with APC-conjugated Staining:Article Title: Cooperation of PD-1 and LAG-3 in the exhaustion of CD4 + and CD8 + T cells during bovine leukemia virus infection Article Snippet: PBMCs were incubated in PBS containing 10% goat serum (Sigma–Aldrich, St. Louis, MO, USA) at room temperature for 15 min to prevent nonspecific reactions. .. Cells were then washed and stained with anti-PD-1 monoclonal antibody (mAb) (5D2, rat IgG 2a ) [ ] or rat IgG 2a isotype control (R35-95, BD Biosciences, San Jose, CA, USA) for 20 min at room temperature, washed with PBS containing 1% bovine serum albumin (BSA; Sigma–Aldrich), and labeled with APC-conjugated |